2.2. Biochemical determinations
For biochemical determinations we used haemolymphatic,
hepatopancreatic, and muscle tissue of the males and females. We
used gonadal tissue only from females, because insufficient
gonadal tissue was obtained from the males. The parameters
measured require about 50 mg of tissue for analysis, and this
amount was impossible to obtain from male gonads. The metabolic
parameters of the tissue sample of each animal were determined
in triplicate using spectrophometric methods.