Fresh leaves of chaplu ,mulberry and rang chuet with average size (6×10 cm.) were obtained from a farm in Pattani. The leaves from all plants were washed, dehydrated in a dry oven
at 50 oC for 15 h and pulverized into coarse powder. The dried plant powder was kept in a polyethylene bag. Each dried plant powder (1%w/v) was extracted with hot distilled water at
95oC for 10 min. The crude plant extract was filtrated and cooled at 4 o
C. Tilapia fillets were dipped with crude extract of each plant (1%w/v)
for 10 min at 4 oC. After treatment, fillets were drained for 10 min. Then fillets of each treatment were placed in pouch. The control samples were soaked in distilled water under the same condition. All samples were taken for microbiological, chemical and sensory analyses every 3 days for up to 15 days.