MR (Methyl Red)
The methyl red test was employed to detect the
production of sufficient acid during the fermentation of
glucose and the maintenance of condition, the pH of a
24hr-old culture was sustained below a value of about
4.5 as shown by a change in colour of the incubation.
The Voges-Proskauer test was ran to test the ability of
the organism to produce acetylmethylcarbinol. The tests
were ran using the commercially prepared MR medium.
Large inoculum of the test organism was
picked and inoculated into the prepared and sterilized
test-tubes. Three [3] drops of methyl red indicator was
added into the culture to the test-tube and the test-tube
was shaken. A red colour indicates a positive reaction
while a yellow colour, indicates a negative result [4].