Different media (BD™ Difco™, Madrid, Spain) and culture
conditions were used for each microorganism group: a) Plate Count
Agar for mesophilic aerobic bacteria, incubated at 30 C for 24 h; b)
McConkey Agar for enterobacteria, incubated aerobically at 37 C
for 24 h; c) Sabouraud Cloranfenicol Agar for yeast, incubated for
24e48 h at 30 C; and d) Rose Bengal Agar for moulds, incubated at
25 C for 3e5 days. The counts were carried out in a colony counter
(Suntex 570 Colony Counter). The results are expressed as the log
CFU g1 ± SD (n ¼ 3).
Different media (BD™ Difco™, Madrid, Spain) and cultureconditions were used for each microorganism group: a) Plate CountAgar for mesophilic aerobic bacteria, incubated at 30 C for 24 h; b)McConkey Agar for enterobacteria, incubated aerobically at 37 Cfor 24 h; c) Sabouraud Cloranfenicol Agar for yeast, incubated for24e48 h at 30 C; and d) Rose Bengal Agar for moulds, incubated at25 C for 3e5 days. The counts were carried out in a colony counter(Suntex 570 Colony Counter). The results are expressed as the logCFU g1 ± SD (n ¼ 3).
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