The concentration
of conidia in each fungi suspensionwas standardized at 108 conidia
per mL using Neubauer chamber and counting in MEA (25 C/5
days) (Delgado et al., 2012a, 2012b; Sant'Ana et al., 2009). Standardized
suspensions were further stored at 2 C. Spore suspensions
were stored for up to 4 months, and periodically checked
through microscopy and enumeration on MEA agar to ensure their
concentration remained stable.