The present study evaluated the effect of monochromatic and combinations of light emitting diode light color on
the performance and behavior of laying hens. At 12 weeks of age, 600 Hy-line Brown pullets were randomly divided
into eight lighting treatments with three replicates of 25 birds in each room. The lightning was set-up as follows;
white (W, control; 16 h/d), red (R; 618-635 nm; 16 h/d), green (G; 515-535 nm; 16 h/d), blue (B; 455-470 nm; 16
h/d), red→green (R→G; 14 h→2 h/d), red→blue (R→B; 14 h→2 h/d), red→green→blue (R→G→B; 12 h→2h→2
h/d) and red→ blue→ green (R→B→G; 12 h→2h→2 h/d) light treatments. The light colors significantly influenced
egg production, which was higher for the combination R→G (89.56%) and monochromatic R (87.34%) and lower in
the G (85.26%) and B (83.75%) light treatments. Conversely, egg weight was remarkably heavier in the B and G light
treatments than those of monochromatic R and combination treatments. Concurrently, better feed conversion ratio
was found for the combination R→G→B and R→G and the monochromatic R light treatment. Egg shells were
significantly pigmented by the combinations of R→B→G and R→G→B. Furthermore, egg shell breaking strength
increased following the G treatment. Higher frequencies of ground pecking, ground scratching, and tail wagging were
observed in the R group and these behaviors were less frequent in the B groups. Birds under G and B lights spent a
longer time perching. These results suggest that a combination of R→G and monochromatic R light enhanced egg
production. In contrast, R light activated the bird’s movement, whereas B light decreased movement, and birds spent
a longer time perching.