An internal control, targeting a nuclear cocoa microsatellite,
was used in a multiplex PCR with the CSSV assay. The single
copymicrosatellitemarkernamedmTcCIR25 (EMIL accession number
Y16997) designed for T. cacao by Lanaud et al. (1999) was
used. The primer sequence for the marker is as follows: forward
primer: CTTCGTAGTGAATGTAGGAG and reverse primer: TTAGGTAGGTAGGGTTATCT.