After each extraction stage, the hexane phase was filtered using a 2–4 μm filter paper in order to eliminate the remaining solid particles and then dried over anhydrous sodium sulfate. Later, hexane was removed using a rotary evaporator at 40 °C under vacuum at 50 mbar and reused for the consecutive stage. Lipids were stored in a desiccator overnight and weighed the next day to determine the extraction yield.