sequences
To identify the nearest phylogenetic neighbors,
each partial sequence of 18S and ITS1 rDNA genes from
yeast strains TB2-1 and TW1-3, was compared to sequences
in the NCBI GenBank database (www.ncbi.nlm.nih.gov) using
the homology search tool (blastn) (Altschul et al., 1997).
To produce phylogenetic trees, sequences were aligned using
the CLUSTAL W multiple sequence alignment program
(version 1.81) (Thompson et al., 1994) and edited manually.
Alignments were performed using several yeasts that were
closely related to yeast strains TB2-1 and TW1-3, based on
information obtained using the BLAST search. Phylogenetic
trees were constructed using MEGA, version 3.1 (Kumar et
al., 2004).
Isolation of Thermotolerant Yeasts