The anesthetic dosage of MS-222 in fish ranges from 50- 200 mg/L. Like many of the anesthetics, there exists a wide
range of species sensitivity. Aeration should be provided in the anesthetic solution as hypoxia is a potential side affect.
Because of its acidic nature, a solution of 100 mg/L has a pH of 5.0 in very soft water. Buffering the stock solution is not
feasible due to formation of a white precipitate. Adjustments must therefore be made after making the appropriate dilution.
The pH of seawater does not appear to be significantly affected by the addition of stock solution.