Fig. 3. Effects of rock bream TRAF2 on NF-kB activity of Epithelioma papulosum cyprini
(EPC) cells harboring NF-kB reporting vector alone (NF-kB), NF-kB reporting vector
plus empty control vector (Empty), or NF-kB reporting vector plus TRAF2 expressing
vector (TRAF2). Cells were grown to 100% confluence and exposed to 1 mg/ml of the
recombinant rock bream TNF-a (Stimulation with TNF-a) or exposed to just phosphate
buffered saline (PBS; No Stimulation). After 12 h (A) and 24 h (B) of the cultivation,
50 ml of the cell culture medium was collected, and analyzed for luciferase activity.
Results are mean þ standard deviation of triplicate samples. Different small letters and
capital letters on the bar represent significantly different at P < 0.05 among groups
not-stimulated and stimulated with TNF-a, respectively.