the population dynamics of Wolbachia in relation to its role in various life cycle stage of Brugia malayi has been studies by Fenn and Blaxter, McGarry et al., and McCall et al. further suggesting that these endosymbionts may play different role in different life cycle stage of filariae. Microfilariae, L2, and L3 of B. malayi have the lowest ratios of Wolbachia to nematode DNA, but there are many Wolbachia clustered in large groups in L4. in female worms, Wolbachia numbers increase further as the worm matures and the ovary and embryonic larvae stages become infected.