Glyphosate (N-phosphonomethylglycine) is the most commonly used herbicide worldwide. Due to the concern regarding its toxicity for non-targeted species in soil, glyphosate-degrading microorganisms in soil is of interest. The developed consortium is potent glyphosate degrader with quick action as indicated by the shorten lag times. The success of this will depend on isolating bacteria with the ability to grow in presence of glyphosate.The samples of soil and water from agriculture that expect to have the use of glyphosate, 80 samples were isolated from bacteria to digest glyphosate effectively with technical continuous culture by adding concentrate of glyphosate are 1,3,6 and 12 g / L . Selection of the 19 isolates of bacteria can grow. When the concentration of glyphosate are 15,18 and 20 g / L and measuring the rate of growth by turbidity found that 10 isolates well grow up . Consequently when the degradation of glyphosate by determination glyphosate reduced after digestion of bacteria by HPLC with a measurement of the growth rate by measuring the turbidity of culture, Testing the optimal conditions for the growth of bacteria can degrade glyphosate in various conditions, including temperature, PH and the concentration of NaCl different. Found that the temperature of 37◦C and 45◦C to made bacteria grow well in this study, all isolates. However, others factors such as PH condition and concentrations of NaCl were studied bacterial isolates grew well in all different when Identification, Five bacterial stains were isolated from different untreated soils of agricultural, the stains were able to grow in a medium containing glyphosate as sole carbon or phosphorus source by enrichment cultures of these soils. Based on bacteria stain were identified though MALDI-TOF MS and biochemical properties . The bast strain amongst them was identified as Klebsiella pneumoniae. This isolate showed the highest growth level in the presence of glyphosate.