Inoculum development
Pure cultures of selected bacterial isolates were individually
maintained on CMC supplemented minimal agar slants at 4
°C, until used. Pure cultures of selected bacterial isolates
were inoculated in broth medium containing 0.03 % MgSO4,
0.2% K2HPO4, 1% glucose, 0.25% (NH4)2SO4 and 1%
peptone at pH 7 for 24h of fermentation period. After 24h of
fermentation period these vegetative cells were used as
inoculum source.