Immunoglobulins from the antiserum to GLRaV-2 used in standard DAS-ELISA (Clark & Adams, 1977) showed a high reactivity with sap from virus-free N. benthamiana (Table 1). The immunoglobulins could therefore not be used for detection of the virus in this host. The same DAS-ELISA, however, could clearly differentiate GLRaV-2-infected and virus-free grapevines