The purpose of this study reported here was to investigate the possibility that whether D. vulgaris subsp. vulgaris (Spear et al., 2000) would be able to use U(VI) as a terminal electron acceptor and immobilize it (Elias and Suflita , 2004) and to perform biochemical testings to find out specific species of Desulfovibrio present in soil samples, collected from corresponding villages, responsible for uranium reduction if they do so. The results indicate that D. vulgaris subsp. vulgaris can enzymatically reduce U (VI) present in water samples of villages of Bathinda, Punjab and presence of uranium reducing members of Desulfovibrio was also reported.