Sections from Group III(G-CSF group) showed that most
renal corpuscles appeared normal with intact glomerular
tufts. Mild peritubular capillary congestion was noted with
mild cytoplasmic vacuolation in some tubular cells. Nearly
all tubular cells had vesicular nuclei (Figures 3A and 3B).
Sections in the medulla showed that some collecting
tubules contain cellular debris or mild cytoplasmic vacuolation
(Figure 3C).
3.2.2. Toluidine-blue-stained sections
Sections from both control subgroups (IA and IB)
showed apparently normal renal corpuscles with narrow
Bowman’s space. The renal tubules had clear basal striations,
prominent brush borders, and large vesicular nuclei
with prominent nucleoli, as well as clear continuous basal
laminae (Figure 4A).Most renaltubules fromtheADRgroup
showed cytoplasmic vacuolation, nonclear basal striations,
partial or complete loss of brush border, as well as many
small irregular nuclei. Epithelial desquamation and interrupted
basal laminae were also seen (Figure 4B). Renal
tubules from the G-CSF group showed clear basal striations,
prominent brush border, and large vesicular nuclei. However,
mild cytoplasmic vacuolation and irregular nuclei
were detected in some cells (Figure 4C).
3.3. Histochemical results
3.3.1. Masson’s trichrome-stained sections
Sections from both control subgroups (IA and IB)
revealed the same findings. The renal cortex showed the
normal distribution of collagen, which appeared as fine collagen
fibers among glomerular capillaries and between the
renal tubules (Figure 5A). Renal cortex from Group II (ADR
group) showed marked deposition of collagen fibers within
the glomeruliinthe renal corpuscles, as well asbetweenthe
renal tubules (Figures 5B and 5C). As for Group III (G-CSF
group), there was mild deposition of collagen fibers in the
renal corpuscle and between the renal tubules (Figure 5D).
3.3.2. PAS-stained sections
The examined renal cortex from both control subgroups
(IA and IB) showed multiple tubules with intact brush