The reverse transcription step was omitted as described previously (Jangam et al., 2009), and the qPCR reactions were run on the Stratagene Mx3005p (Agilent Technologies, Santa Clara, CA) using the FAM and Cy5 channels for data collection rather than the Abbott's automated system, m2000sp™ and m2000rt ™ (Tang et al., 2007).