Low-energy ion beam biotechnology has achieved tremendous successes in inducing crop mutation and
gene transfer. However, mechanisms involved in the related processes are not yet well understood. In
ion-beam-induced mutation, ion-bombardment-produced X-ray has been proposed to be one of the secondary
mutation sources, but the speculation has not yet been experimentally tested. We carried out this
investigation to test whether the low-energy ion-beam-produced X-ray was a source of ion-beaminduced
mutation. In the investigation, X-ray emission from 29-keV nitrogen- or argon- ion beam bombarded
bacterial Escherichia coli (E. coli) cells held in a metal or plastic sample holder was in situ detected
using a highly sensitive X-ray detector. The ion beam bombarded bacterial cells held in different material
holders were observed for mutation induction. The results led to a conclusion that secondary X-ray emitted
from ion-beam-bombarded biological living materials themselves was not a, or at least a negligible,
mutational source, but the ion-beam-induced X-ray emission from the metal that made the sample
holder could be a source of mutation.
Low-energy ion beam biotechnology has achieved tremendous successes in inducing crop mutation andgene transfer. However, mechanisms involved in the related processes are not yet well understood. Inion-beam-induced mutation, ion-bombardment-produced X-ray has been proposed to be one of the secondarymutation sources, but the speculation has not yet been experimentally tested. We carried out thisinvestigation to test whether the low-energy ion-beam-produced X-ray was a source of ion-beaminducedmutation. In the investigation, X-ray emission from 29-keV nitrogen- or argon- ion beam bombardedbacterial Escherichia coli (E. coli) cells held in a metal or plastic sample holder was in situ detectedusing a highly sensitive X-ray detector. The ion beam bombarded bacterial cells held in different materialholders were observed for mutation induction. The results led to a conclusion that secondary X-ray emittedfrom ion-beam-bombarded biological living materials themselves was not a, or at least a negligible,mutational source, but the ion-beam-induced X-ray emission from the metal that made the sampleholder could be a source of mutation.
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