- Oil from spent coffee grounds was extracted with supercritical carbon dioxide.
- An oil extraction yield of 90% was achieved at a CO2/SCG mass ratio of 35 kg kg1 .
- Cupriavidus necator was cultivated with SCG oil as sole carbon source.
- The cells accumulated up to 78% (w/w) poly-3-hydroxybutyrate.
- The polymer exhibited similar properties to other P(3HB) biopolymers.