Spore suspension preparation (inoculum)
The E. turcicum isolate DI was grown on V-8 agar for 7 d. Spore
suspensions were prepared by scraping spores from the agar surface with an
L-shaped glass rod and filtered through two layers of cheese cloth. The number
of spores was determined using a haemacytometer and the concentration was
adjusted to 105 spore/ml.