Confocal laser scanning microscopy (CLSM) was used to observe the nanocapsule morphology and deposition mechanism of chitosan
and CMC on the surface of NEI and NEII droplets, respectively
Chitosan and CMC layers were used to stabilize the inner oily phase of the nanocapsule.
3 lL solution of Nile Red fluorescent dye (0.02%, w/v) was added to 500 lL of each sample (NEII and nanocapsule suspension) to mark the oil.