Experiments were conducted for the standardization of in vitro culture technique for the mass propagation of Musa sp is one of the important fruit crops. Shoot tips were used as explants and they were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP, Kin and IAA both in individual and in combined form for shoot inductions and the best results were obtained from MS medium supplemented with BAP+IAA at the concentration of 3.0mg/l and 0.5mg/l respectively. Best root formation of in vitro developed shoots could be achieved on halt strength MS medium supplemented with IBA at concentration 1.0mg/l. The in vitro developed plantlets were transferred to pot and they were grown in greenhouse for hardening and finally they were planted in the open field. Around 87% of plants were successfully established in natural field condition.