the activity of the nanobiocatalysts decreased. This seems to indicate that as the nanoassembly gets thicker, the benefit of the extra fGO layer in inducing a more active enzyme conformation is outweighed by a reduced substrate availability when the enzyme is located in an internal layer of the nanoassembly. It is worthy to note that the addition of more than 3 fGO layers reduces the dispersion of the material in the reaction medium, which in turn could lead to a decrease of the catalytic activity as observed for fGO-TvL-fGO-TvL-fGO-Tv-fGO.