The main aim of designing tree-shaped or Y-shaped pattern is to integrate a self-calibration on the test strip. The pattern of the test sheet can be flexibly designed according to the need and requirement of the determination of the analyte. For detecting multiplex
concentration of standard glucose solutions, tree-shaped strip has the advantages because it can assure the uniformity of microflows in different branches and the sample information can be collected at the same time. In our work, the main purpose of tree-shaped strip
was to obtain the calibration curves.