2.6. Statistical MethodsResults were expressed as mean ± S.D. Variance homogeneity was tested by the Hartley test. All data were considered statistically significant when the probability of Type I error was <0.05. The effect of supplementation on body weight, diet and BCAA intake was evaluated after one hour of swimming (1H) and also after the swimming exhaustion test (EX) by one way ANOVA followed by the Tukey HSD test. When variance homogeneity was not observed, data were evaluated by the Kruskal-Wallis ANOVA test. Except for two variables (liver and gastrocnemius muscle glycogen concentrations) that did not show variance homogeneity, mixed design factorial ANOVA was applied to analyze the biochemical variables considering as independent factors the supplementation at three levels (−1 = AIN-93 M, 0 = AIN-93 M + 3.57% BCAA and +1 = AIN-93 M + 4.76% BCAA), and the time of exercise before the sacrifice at two levels (−1 = 1 h and +1 = exhaustion). All calculations and graphs were made using the Statistica 7.1 software [33] (StatSoft Inc., Tulsa, OK, USA).- See more at: http://www.mdpi.com/2072-6643/4/11/1767/htm#sthash.zANTqsIh.dpuf
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