Studies carried by Ozguven et al. (2008) and Singh (2000) have shown positive effect on artemisinin content and yield by the application of varying nitrogen doses. But to our knowledge, no study has been conducted so far to study the effect of organic manure and interactive effect of chemical fertilizers (N, P, K and S) on A. annua L. plants. We therefore, conducted an experiment with the objectives to compare the effects of organic manure and chemical fertilizers on artemisinin contents, biomass accumulation in various plant parts and artemisinin yield through phenological stages of A. annua L. plants.