Yeast cells were grown aerobically in 80 mL of a seed medium containing 0.8 g of yeast extract, 1.6 g of polypeptone, and 1.6 g of glucose at 30 o C for 24 h with shaking (2.5 Hz). The cells (about 150 mg as dry matter) recovered from 25 mL of the seed medium were inoculated to 50 mL of a fermentation medium containing 10 g of sugars in the form of thick juice and whey,
0.1 g of NH4H2PO4, and 0.025 g of MgSO4 7H2O in a 100 mL