Twenty four hours urine and heparinized blood samples were collected from the patients for biochemical analysis. Urine samples were subjected to a battery of chemical tests to detect metabolic disorders.
The aminoacids were separated by the paper chromatography technique of Sharma and Zweig (1971) (6) for qualitative analysis. Quantitation of amino acids in urine was done on automatic amino acid analyser (LKB4400 model) using lithium buffer system