Fatty acids methyl esters (FAMEs) were prepared in accordance
with the method developed by Prescha, Swiedrych, Biernat, and
Szopa (2001). The separation of FAMEs was performed on gas
chromatograph 6890N (Agilent Technologies, USA) equipped with
a FID detector and the capillary column SP-2560
100 m 0.25 mm 0.2 lm (Supelco, Sigma Aldrich). Hydrogen
was used as a carrier gas at a flow rate of 1.5 ml/min and the
separation was carried out at a temperature set from 110 C (for
5 min) to 220 C (for 15 min); the temperature increased at a rate
of 2 C/min. The identification of particular fatty acids was accomplished
by a comparison with the external standards and
Chemstation v. B.04.02 was engaged to calculate the results.