an adoptedin vitroassay that is used for identification of water soluble
ocul i d i it tarcorrosivesandsevere irritants
perform in a well where a confluent monolayer of Madin-Darby Canine
Kidney (MDCK) is used as a separation between two chambers and
uses a fluorescein dye as marker
test substance with potential to impair the junctions of the MDCK cells
increase permeability and then the, the fluorescein passes through thefluorescein passes through the
monolayer and the fluorescein leakage (FL) increases
combine with otherin vitrotests for evaluation of eye irritation potential of substances