2.7. Data analysis
Origin software version 7.5 (OriginLab Corporation, Northampton, USA) was used for data analysis. Data were subjected to analysis of variance (ANOVA) and significant differences among the means were determined by Fisher Post hoc test at a p b 0.05 significant level. All results were expressed as the mean ± standard deviation. D10-values in kGy, which is the irradiating dose required to reduce microorganisms by 90%, were calculated from the linear regression model of the log of the surviving fractions.