2.1.1. Case study 1: genetic structures in natural
forests and plantations of Dalbergia sissoo in Nepal
The natural distribution area of D. sissoo stretches
on the foothills of the Himalayas from Afghanistan to
Myanmar. The species is widely planted throughout its
natural distribution and in many other tropical areas.
Genetic variation of D. sissoo was investigated in a
transect consisting of five population pairs in Nepal, each pair consisting of one natural population and one
neighbouring plantation of unknown origin. Variation
patterns of presumably maternally inherited cpDNA
were assessed by PCR-RFLP and cpSSR techniques
(five individuals/population); isozymes were used to
characterize variation at biparentally inherited nuclear
genes (50 individuals/population). Eight cpDNA haplotypes
were observed, and the expected hetero-zygosity
at the isozyme gene locus Gdh-A was calculated