Calculation:- Count 4 corner squares and calculate the average. Each large square of the hemocytometer, with cover slip in place, represents a total volume of 0.1 mm3 (1.0mm X1.0mmX 0.1mm) or 10-4 cm3. Since 1 cm3 is equivalent to approximately 1 ml, the total number of cells per ml will be determined using the following calculations: