In the second selection scheme, seeds of the three bulk BC2F2
populations were sown in the seedling nursery at the CAAS
experimental station in Beijing on May 10, 2010. On June 4, 480
25-day old BC2F2 seedlings from each population were
transplanted into a 40-row plotwith 3 rows ofHHZ(the recipient inserted in every 10 rows as the checks. The field was managed
using standard practices under normal irrigated conditions. At
maturity, high yielding (HY) plants were visually identified and
harvested and dried naturally for 10 days prior to measuring
grain yield. Plants with at least 10% higher yield than HHZ were
selected, resulting in 26, 16 and 22 HY plants from the HHZ/IR64,
HHZ/AT354 and HHZ/C418 populations.