all suspended in 1 ml phosphate buffer saline (PBS, pH 7.4). Blood was collected on days 0, 14 and 21 and serum anti-HEWL antibody
was assessed by ELISA. On day 21, cows were injected intradermally with candin and/or CaCW and PBS (negative control). Skin measurements were taken
at 0 (day 21), 24 and 48 h post-intradermal injection. (a) Anti-HEWL antibody responses at days 14 and 21. (b) DTH responses to candin and CaWC at 24 and
48 h post-intradermal injections. Probability values are reported as p
≤
0.05. All responses were significantly different from 0. Dissimilar letters indicate
significantly different responses between points of time.