A PMI experiment was performed by disposing
a burned pig in the woods. Maggots and flies were
collected for species identification two times each
day. For confirmation, the DNA was extracted from
adult flies for mtDNA sequence analysis at the cytochrome oxidase b subunit I between nucleotide
position 2495 and 2800. Coincidentally, a homicide case occurred at a location 6 kilometers away
from the experimental site. Maggots were collected
from the victim’s body that had been burned and
then disposed of. In this case, PMI was successfully
pinpointed by referring to the pig study that had
been carried out under similar circumstances.