Ethanol extracts (80 ml) were loaded onto sterile filter
paper discs (+ 8 mm, Advantec, Tokyo, Japan) twice
with air-drying in between. Control discs were similarly
prepared using distilled water and 100% ethanol. Each
of the discs was placed on the aforementioned bacterial
culture plates and incubated at 35 1C for 18–24 h.
Inhibition zones (including the diameter of disc) were
measured and recorded.