Jatropha curcas as potential biodiesel feedstock is difficult to propagate through tissue culture. The research
development for Jatropha is massive nowadays and propagation can be conducted through conventional or nonconventional
techniques in biotechnology. Biotechnology approach through organogenesis and embryogenesis
pathways is needed. The objectives of the research were to obtain in vitro culture media optimization through
embryogenesis pathway. Plant materials used in this experiment were embryo axis and cotyledon to obtain somatic
embryo. The result of the experiment showed that somatic embryos were obtained only from MS medium
supplemented with picloram 1.0 mg/l for both embryo axis and cotyledon explant.