Sixty-four pigs, reared from 21 to 35 d of age, were fed eight different diets according to a 2 4 factorial design: a control diet or the control diet supplemented with three different levels of B. animalis (107, 109, 1011 colony-forming units/d), crossed with 0% or 2% sugar beet fructo-oligosaccharides. Pigs were then sacrificed, and the jejunum mucosa, ileocecal lymph nodes, and liver were sampled to determine the presence of Bifidobacterium spp. DNA and to quantify the expression of TLR2-, TLR4-, and tumor necrosis factor-– encoding genes