Breeding of the target genotype line
Using selected markers (Table 1), genotypes of the 525 RILs with PGC correlative loci were analyzed. Twenty RILs (RIL1-RIL20) were selected, and their genotype and agronomic traits profiles were investigated (Tables 1 and 2). Low PGC RILs were not identical to Minhui 63 with regard to all of the PGC loci genotype, but they were similar to the primary Minhui 63 genotype. Because Minhui 63’s PGC was 6.5%, if the RILs’ PGC reached 0%, the RILs needed some alleles from Zhenshan 97, which reduced the PGC.