To bind Abs covalently and in an oriented manner the carbohydrates
chains from the Fc regions of Abs specific for HRP were
reduced and linked to the amino groups of the positive polyelectrolyte
PAH forming the outer PC shell. To demonstrate that the
Abs were correctly attached onto the PC surface, Ab-PCs were incubated
with HRP with a subsequently three-step magnetic separation
and washing with buffer. With the aim of detecting the
amount of functional HRP extracted with the Ab-PCs, we proved
the catalytic properties of HRP.