Fig. 3 Western blot analysis of MMLRaV CP using a healthy
mulberry sample as a negative control (1) and a partially purified
virus sample (2). Pre-stained proteins (Fermentas, Lithuania) were
used as size markers (MW). The healthy mulberry sample was
processed in the same ways as the sample shown in lane 2. An
antiserum produced using the prokaryotic expression product of the
partial CP gene of MMLRaV (a 38-kDa protein encoded by the 30 half
of the MMLRaV CP gene) for injecting rabbits at Jiangsu University,
China, was used as the primary antibody. HRP (horseradish peroxidase)-
conjugated goat anti-rabbit IgG (Beyotime) was used as the
secondary antibody. The immuno-reaction was visualized with DAB
(3,30-diaminobenzidine) as substrate. The arrow indicates the position
of the CP band. The additional faint band may correspond to a
degradation product of the MMLRaV CP