Using degenerate primers targeting a fragment of this gene , a PCR - amplification product of the expected size (432-bp) was obtained from all isolates in ARDRA cluster l (pantoea) , lll (achromobacter) , lv (microbacterium) ,vll(sphingomonas) ,lx-1 to-3 (rhizobium), xl (micrococcus) , xll-1 and xlll (pseudomonas).