• Finally, the entropy and %coverage values (Fig. 5, columns U and V) were summarised in a chart
where the entropy was drawn as column diagram (blue) related to the main y axis while the %
coverage was shown as line graph (green) related to the secondary y axis.
• The graph clearly shows the presence and distribution of the most variable (the highest columns)
and also the conserved positions (Hi converging to zero ) suitable for oligonucleotide selection.