For thermal treatments, 0.07 mL of LWE with or without each additive and 109 CFU/mL of the bacterial suspension was added to 0.63 mL of the same medium (LWE with or without each additive) tempered at 60 ± 0.2 °C (FX Incubator, A.F. Ingenieria S.L., Valencia, Spain). For PEF followed by heat treatments, 0.07 mL of the 0.32 mL PEF-treated LWE (with or without each additive) was added to 0.63 mL of the same medium tempered at 60 ± 0.2 °C. The actual temperature was
controlled with a thermocouple wire introduced in a 0.7 ml LWE test tube inside the incubator.