The first was an E. coli resistant strain S01, belonging to phylogroup F, isolated from ductus deferens of a rooster during the current research and carrying blaCTX-M and blaTEM genes, the second strain was the standard sensitive E. coli ATCC®25922 belonging to phylo-group B2. Bacterial suspensions of 108 CFU/mL were prepared for each isolate using a 0.5 McFarland standard.