The Applied Biosystems capillary
electrophoresis platforms, the 3130
Series and 3730 series Analyzers, in
conjunction with GeneMapper®
Software v3.7 or higher, provide an
optimal solution for performing
T-RFLP assays. Using this system we
were successfully able to perform
analysis of microbial samples that had
been isolated from PRB enrichment
bioreactors. System features include
high resolution, excellent sizing
precision, low turnaround times,
and ease of use. The integration of
GeneMapper software with these
instruments provides one-button
operation for sample loading, datacollection, fragment size-calling, and
accurate marker scoring in both
graphical and standard binary formats.
All of these features enable the
generation of ample high-quality data
with minimal hands-on time required
and little need for manual data review.