Anthurium antioquiense Engl. is a native plant belonging to
the Araceae family. It grows on rocks in clear-water rivers and
well-protected zones, similar to the waters in certain watersheds
of the Antioquia Department, Colombia. Loss of habitat has
threatened this promising ornamental plant species, which is
also important because of its role in the ecosystem. In vitro tissue
culture is considered an efficient alternative for the propagation
of endangered species with the aim of establishing short-,
medium- and long-term conservation programs. In the present
research, in vitro introduction and shoot induction from A.
antioquiense seedlings were performed. The highest production
of shoots was obtained in a ½ MS (half-salt content) medium
with 1 mg L-1 of BAP, which attained a 23.7 shoots/explant
per month multiplication rate. The in vitro plants generated
from shoots were individualized and transferred to a growth
regulator-free medium. Rooting did not require the presence
of growth regulators, and the adaptation of the in vitro plants
to ex vitro conditions achieved a 98% survival rate.