Filtration format
Reagents available from PerkinElmer:
Radiolabeled thymidine (see next section)
TC-treated microplates for cell culturing (we recommend CulturPlates™)
Glass fiber filters (UniFilter plates or Filtermat)
Scintillation cocktail or Meltilex® solid scintillant (Cat. No. 1450-441) (if using Filtermat)
Scintillation vials (if using filter discs)
Cell harvester
TopSeal-A™ (Cat. No. 6050195) and BackSeal (Cat. No. 6005199) (if using UniFilter plates)
Radiometric detector (such as a Tri-Carb® liquid scintillation counter, or a high-throughput detector such as a TopCount® or MicroBeta®)
Reagents available from other suppliers:
Stimulation factors
Trypsin
Wash buffer (e.g. PBS)
Cytostar-T format
Cytostar-T plates (Cat. No. RPNQ0162 for 96-well, RPNQ0165 for 384-well)
TopSeal-A™ adhesive plate seal (Cat. No. 6050195)
White backseal, if counting from the top (Cat. No. 6005199)
Radiolabeled thymidine (see next section)
High-throughput radiometric detector (such as a TopCount or MicroBeta)
Cell culture medium, buffer for labeling
Stimulation factors
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